Organisation/Company: CNRS
Department: Institut de Recherche en Infectiologie de Montpellier
Research Field: Chemistry » Biochemistry, Physics » Biophysics, Biological sciences » Biological engineering
Researcher Profile: Recognised Researcher (R2)
Country: France
Application Deadline: 9 Dec 2024 - 00:00 (UTC)
Type of Contract: Temporary
Job Status: Full-time
Hours Per Week: 35
Offer Starting Date: 1 Feb 2025
Is the job funded through the EU Research Framework Programme? Not funded by a EU programme
Is the Job related to staff position within a Research Infrastructure? No
Offer Description The PostDoctoral fellow will study the assembly of RSV (Respiratory Syncytial Virus), focusing on the interactions of the RSV M protein with lipids and other proteins involved in viral particle formation:
In vitro on biomimetic lipid membranes (LUV/SLB)
Ex vivo in lung cell lines using fluorescence microscopy in living cells or super-resolution imaging (confocal/STED).
He/She will interact with the consortium involved in this project and will:
Manufacture biomimetic membranes to study the interactions of the M protein and mutants with LUV or SLB (M protein and mutants provided by a partner of the consortium).
Produce DNA plasmids in bacteria.
Perform cell culture in BSL2 and transfection.
Study the localization of M and other fluorescent viral proteins in lung cells over time (microscopy of living organisms and confocal microscopy/STED by immuno-fluorescence).
Present results in lab meetings and national/international conferences.
Travel nationally to other labs of the consortium.
Speak English.
Exhibit team spirit.
Work in BSL1, BSL2 (cell culture), and BSL3 environments.
The postdoctoral researcher will work at the CNRS in Montpellier at IRIM (Infectiology Research Institute of Montpellier) in the MDVA team (Membrane Domains and Viral Assembly). This team focuses on the assembly of enveloped RNA viruses and the role of interactions between viral structural proteins, viral RNA, and host cell lipids using virology, biochemistry, cell biology, and super-resolution microscopy techniques. The team currently includes 3 researchers (in virology, biophysics, and molecular biology), an engineer (in cell biology), 2 Post-Doctoral researchers, 4 Doctoral students, and a Master's degree student, making it an interdisciplinary and international team.
Additional Information Eligibility criteria:
Lipid biochemistry
Protein-Lipid interaction
Cell culture
Microscopies (at least confocal)
Virology is a plus
Membrane biophysics is a plus
English B1/B2 is required
Additional comments:
This RSV-Mlipo research project is funded by the French ANR agency, with a national consortium involving 3 institutes: IRIM in Montpellier, IBS in Grenoble, and INRAE in Jouy-en-Josas (from 2024 to 2027).
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