Organisation/Company: CNRS
Department: Expression Génétique Microbienne
Research Field: Biological sciences » Biology
Researcher Profile: First Stage Researcher (R1)
Country: France
Application Deadline: 3 Dec 2024 - 00:00 (UTC)
Type of Contract: Temporary
Job Status: Full-time
Hours Per Week: 35
Offer Starting Date: 3 Feb 2025
Is the job funded through the EU Research Framework Programme? Not funded by a EU programme
Is the Job related to staff position within a Research Infrastructure? No
Offer Description
This contract is part of a project funded by the National Research Agency (ANR) aimed at studying the role of ABC-F proteins in the translation process in the cyanobacterium Synechocystis sp. PCC 6803. The recruited post-doctoral researcher will be part of the 'Physiology and Regulation of Protein Synthesis' team led by Grégory Boël at the Institute of Physical and Chemical Biology (IBPC). He/She will work under the supervision of Laura Monlezun.
The project aims at characterizing the physiological role of the two translation factors from the ABC-F family (Fostier et al. FEMS Letters 2021) in the cyanobacterium Synechocystis PCC 6803. The candidate will be responsible for the transcriptomic (RNA-seq) and proteomic (label-free MS) analysis of the different strains. As part of these global approaches, they will also be responsible for the implementation and optimization of the ribosome profiling technique (Ribo-seq) in this organism (Karlsen et al., mSystems 2018), to determine ribosome pauses during elongation, induced in the absence of each ABC-F. Furthermore, the candidate will contribute to elucidating the precise mechanism of action of these ABC-F proteins with respect to target mRNAs and ribosomes through both in vivo and in vitro approaches. They may also be required to supervise Master's level students for some of these experiments.
The candidate will conduct their research within G. Boël's team in the 'Microbial Gene Expression' unit (UMR8261) at the IBPC. The IBPC is a multidisciplinary institute of the National Center for Scientific Research (CNRS) located in central Paris on the Pierre and Marie Campus (5th arrondissement). The institute has notably a proteomics platform and a bioinformatics platform, both of which will be used for this project.
We are looking for a curious, highly motivated candidate with a PhD in microbiology, with demonstrated expertise in cyanobacteria culture and genetics. The candidate should be proficient in molecular biology techniques (cloning, mutagenesis, etc.). Familiarity with the preparation and/or analysis of RNA samples using high-throughput sequencing (mRNA-seq and/or ribo-seq) is preferred. Ideally, experience in protein purification and in Northern Blot and/or Western Blot analysis is desired. A general understanding of protein synthesis and RNA biochemistry is essential.
The candidate must demonstrate autonomy, rigor, and organizational skills in their experiments. Collaborative and teamwork abilities, as well as the capacity to present research findings orally or in writing, are also essential criteria for this position. Lastly, a strong proficiency in both spoken and written English is highly recommended.
Candidates are invited to submit their CV with a full list of publications, a cover letter, and three references who can be contacted, on the CNRS Job Portal.
#J-18808-Ljbffr