Organisation/Company: CNRS
Department: Chimie et Biologie des Membranes et des Nanoobjets
Research Field: Chemistry » Biochemistry, Physics » Biophysics, Biological sciences » Biological engineering
Researcher Profile: Established Researcher (R3)
Country: France
Application Deadline: 28 Nov 2024 - 23:59 (UTC)
Type of Contract: Temporary
Job Status: Full-time
Hours Per Week: 35
Offer Starting Date: 15 Jan 2025
Is the job funded through the EU Research Framework Programme? Not funded by a EU programme
Is the Job related to staff position within a Research Infrastructure? No
Offer Description A post-doctoral researcher position in the molecular mechanisms of bacterial secretion systems under control of the second messenger c-di-GMP. Bacterial secretion systems are membrane macrocomplexes that play an important role in biofilm formation, bacterial cell physiology and pathogenicity, and are important targets in the development of new anti-infective drugs. This project aims to understand the molecular mechanisms of the function and the pharmacology of these macromolecular systems.
This position is assigned to the CBMN laboratory (UMR5248), within the team of Dr. Petya Krasteva whose theme is "Structural Biology of Biofilms".
Responsibilities Development of protocols for the expression and purification of proteins and membrane, cytosolic and/or periplasmic complexes in different heterologous systems, including small and large scale cultures.
Development and realization of solubilization and protein stability tests, as well as functional assays.
Perform protein crystallization screens and contribute to the design of crystal optimization methods.
Data collection at synchrotron facilities in France and abroad (X-ray diffraction and state-of-the-art cryo-electron microscopy (cryoEM)).
Preparation of samples for cryo-EM, in-house data collection and analysis.
Analysis of the scientific literature, preparation of presentations, scientific communication.
The offer is for a 15-month contract funded on an ANR project with possibility to extend depending on the recruitee's performance.
Eligibility Criteria Recommended skills:
Production and purification of plasmid DNA in milligram quantities.
Cloning, mutagenesis, design of expression constructs and molecular biology protocols.
(Membrane) protein purification, including high-throughput solubilization screens and liquid chromatography techniques.
Protein biochemical characterization including knowledge in enzymology.
Protein crystallization, including experience with high-throughput crystallization screening robots and crystal optimization techniques.
X-ray diffraction data collection and structure determination.
Experience with single-particle electron microscopy, including cryo-EM.
Experience with cryo-EM data processing.
Experience in training students and postdocs.
Experience in organizing travels to synchrotrons or other facilities for data collection, as well as handling diffraction/cryo-EM data.
Training in the above will be provided. Experience is preferable but not obligatory.
Additional Comments This is an equal opportunity employment. Researchers from under-represented groups are encouraged to apply.
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